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What tests are done for male fertility?

Male fertility testing primarily involves semen analysis, which measures sperm count (concentration), motility (movement), and morphology (shape). Additional tests may include hormone blood tests (FSH, LH, testosterone), DNA fragmentation testing, and physical examination. Abstain from ejaculation for 2-5 days before testing.

Last updated: December 2025

Male Fertility Testing Guide

Male factor infertility contributes to 40-50% of all infertility cases. Understanding what tests are available and what the results mean can help you take an active role in your fertility journey. Here's everything you need to know about male fertility testing.

Semen Analysis: The Primary Test

A semen analysis is the cornerstone of male fertility testing. It evaluates several key parameters of sperm health:

ParameterNormal Range (WHO)What It Measures
Volume1.5 mL or moreTotal amount of semen in the sample
Concentration16 million/mL or moreNumber of sperm per milliliter
Total Count39 million or moreTotal number of sperm in the ejaculate
Progressive Motility32% or morePercentage swimming forward
Total Motility40% or morePercentage showing any movement
Morphology (Kruger)4% or morePercentage with normal shape
pH7.2 - 8.0Acidity level of semen
Vitality58% or more alivePercentage of living sperm

How to Prepare for Your Test

Before the Test

  • • Abstain from ejaculation for 2-5 days
  • • Avoid alcohol for 2-3 days
  • • Skip hot tubs and saunas for a week
  • • Avoid fever-inducing illnesses
  • • Tell your doctor about any medications

Day of Collection

  • • Wash hands thoroughly before
  • • Use only clinic-provided containers
  • • Don't use lubricants or saliva
  • • Collect the entire sample
  • • Deliver within 1 hour if collected at home

Additional Testing

If semen analysis shows abnormalities, or if you're proceeding with IVF, additional tests may be recommended:

Hormone Testing

Evaluates hormonal causes of low sperm production

FSHLHTestosteroneProlactinEstradiol

DNA Fragmentation

Measures damage to sperm DNA that can affect embryo development

SCSATUNELComet assay

Anti-Sperm Antibodies

Detects immune system attacking sperm

MAR testImmunobead test

Physical Examination

Identifies physical abnormalities affecting fertility

Testicular examVaricocele checkUltrasound

Genetic Testing

Screens for genetic causes of azoospermia or severe issues

KaryotypeY-chromosome microdeletionCFTR gene

Understanding Your Results

Normal Results

All parameters meet WHO criteria. Male factor infertility is unlikely, though one normal test doesn't guarantee fertility. Other factors can still affect conception.

Borderline Results

Some parameters slightly below normal. Usually repeated in 2-4 weeks since sperm quality varies. Lifestyle improvements may help.

Abnormal Results

Significantly low counts or other issues. Further testing and possible treatment referral to a urologist. IVF with ICSI may be recommended.

Medical Terms Explained

Oligozoospermia

Low sperm count

Asthenozoospermia

Poor sperm motility

Teratozoospermia

Abnormal sperm shape

Azoospermia

No sperm in ejaculate

OAT Syndrome

Low count, motility, and morphology

Varicocele

Enlarged veins in scrotum affecting sperm

Track Your Fertility Testing Results

Use IVFPath to store and track your semen analysis results over time. Monitor improvements as you make lifestyle changes.

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Frequently Asked Questions

Medical Review: IVFPath Medical Content Team • Last reviewed: December 2025

Disclaimer: This content is for informational purposes only. Always discuss test results with your doctor or reproductive urologist who can provide personalised advice based on your complete medical history.